Ntau khub ntawm disulfide bonds

Disulfide bonds yog ib feem tseem ceeb ntawm peb - qauv ntawm ntau cov proteins. Peb tuaj yeem pom covalent bonds nyob rau hauv yuav luag tag nrho cov extracellular peptide thiab protein molecules. Ib daim ntawv cog lus disulfide yog tsim thaum cov sulfur atom ntawm ib lub cysteine ​​tsim ib daim ntawv cog lus nrog cov sulfur atom ntawm lwm cov cysteine ​​nyob ntawm qhov chaw sib txawv ntawm cov protein. Cov ntawv cog lus no pab kom ruaj khov ntawm cov protein, tshwj xeeb tshaj yog cov uas zais los ntawm cov hlwb.

Yog tias cov polypeptide tsuas muaj 1 khub Cys, qhov tsim ntawm daim ntawv cog lus disulfide yog qhov yooj yim. Peptides yog synthesized nyob rau hauv cov khoom theem los yog kua theem thiab ces pH8-9 oxidation yog nqa tawm nyob rau hauv cov tshuaj.

Thaum ob lossis ntau khub ntawm disulfide bonds yuav tsum tau tsim, cov txheej txheem synthesis yog qhov nyuaj. Txawm hais tias qhov tsim ntawm disulfide daim ntawv cog lus feem ntau ua tiav thaum kawg ntawm cov txheej txheem hluavtaws, nws qee zaum muaj txiaj ntsig zoo los qhia ua ntej cov disulfides rau conjugate lossis txuas ntxiv cov saw peptide. Feem ntau siv sulfhydryl tiv thaiv pawg yog trt, Acm, Mmt, tBu, Bzl, Mob, Tmob thiab lwm pab pawg. Peb sau ob hom raws li nrog 2- Cl resin thiab Rink Txoj kev tsim ntawm ntau khub ntawm disulfide bonds ntawm polypeptides synthesized siv resin ua tus cab kuj: